Application

Western blot: 1 µg/mL

Immunocytochemistry: 0.5-1.0 µg/mL

Immunohistochemistry on 4% paraformaldehyde fixed tissue: 0.5- 1.0 µg/mL

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Anti-CASK Antibody, clone K56A is an antibody against CASK for use in WB, IC, IH.

Research Sub CategorySynapse & Synaptic Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

CASK-GST fusion protein (amino acids 318-415).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Format: Purified

Specificity

CASK. The immunogen protein represents a CASK-specific domain that lies between the calmodulin binding and PDZ domains. MAB5230 exhibits a specific reaction to the two alternatively spliced isoforms of CASK present in the mammalian CNS, the CASK-B or long (~900 amino acid) isoform present in rat brain membrane preparations, and the soluble CASK-A or short (~600 amino acid) isoform.CASK is a synapse-associated Ca2+/calmodulin-dependent protein kinase that also contains PDZ, SH3 and guanylate kinase domains. CASK is thought to play a critical role in synaptic vesicle exocytosis and synaptic junctions.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots up to 6 months.